@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix nalt: <https://lod.nal.usda.gov/nalt/> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix dc: <http://purl.org/dc/terms/> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix owl: <http://www.w3.org/2002/07/owl#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix agrovoc: <http://aims.fao.org/aos/agrovoc/> .
@prefix ns0: <https://lod.nal.usda.gov/naltv#> .

<https://lod.nal.usda.gov/naltv#marc001> rdfs:label "Field 001 in MARC Authority Format"@en .
nalt:8124_def
  a <https://lod.nal.usda.gov/naltv#Definition> ;
  rdf:value "In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers)."@en, "Método in vitro para producir grandes cantidades de fragmentos específicos de ADN o ARN de longitud y secuencia definidas a partir de pequeñas cantidades de secuencia flanqueantes del oligonucleótido (primers)."@es ;
  dc:source "Medical Subject Headings" .

<https://lod.nal.usda.gov/nalt>
  a skos:ConceptScheme ;
  rdfs:label "NALT Full"@en .

<https://lod.nal.usda.gov/naltv#Topic>
  a owl:Class ;
  rdfs:label "Topic"@en .

nalt:8124
  a skos:Concept, <https://lod.nal.usda.gov/naltv#Topic> ;
  dc:created "2006-01-19"^^xsd:date ;
  dc:modified "2020-09-11"^^xsd:date ;
  skos:altLabel "multiplex polymerase chain reaction"@en, "DNA amplification (in vitro)"@en, "DNA amplification technique"@en, "PCR (genetic technique)"@en, "gene amplification (technique)"@en, "amplification (genetic technique)"@en, "RNA amplification (in vitro)"@en ;
  skos:broader nalt:17409 ;
  skos:definition nalt:8124_def ;
  skos:exactMatch <http://id.cabi.org/cabt/93070>, <http://id.loc.gov/authorities/subjects/sh89002539>, <http://id.agrisemantics.org/gacs/C306>, agrovoc:c_34079 ;
  skos:inScheme <https://lod.nal.usda.gov/nalt>, <https://lod.nal.usda.gov/nalt-core>, <https://lod.nal.usda.gov/nalt-awic> ;
  skos:narrower nalt:196301, nalt:59136, nalt:4815, nalt:226519, nalt:59137, nalt:207911, nalt:197967, nalt:200653, nalt:59138 ;
  skos:prefLabel "polymerase chain reaction"@en, "reacción en cadena de la polimerasa"@es ;
  skos:related nalt:34216, nalt:343052, nalt:292387, nalt:275963, nalt:275961, nalt:312563, nalt:209544, nalt:15977 ;
  ns0:marc001 "29573" .

nalt:196301
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "polimorfismos para la amplificación de regiones blanco"@es, "target region amplification polymorphism"@en .

nalt:59136
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "random amplified polymorphic DNA technique"@en, "técnica de ADN polimórfico amplificado al azar"@es .

nalt:4815
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "polimorfismo de longitud de fragmentos amplificados"@es, "amplified fragment length polymorphism"@en .

nalt:59138
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "simple sequence length polymorphism"@en, "polimorfismo de repeticiones de secuencia simple"@es .

nalt:200653
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "genome walking"@en, "paseo genómico"@es .

nalt:197967
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "amplificación rápida de los extremos de ADNc"@es, "rapid amplification of cDNA ends"@en .

nalt:207911
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "reacción en cadena de la polimerasa cuantitativa"@es, "quantitative polymerase chain reaction"@en .

nalt:59137
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "reverse transcriptase polymerase chain reaction"@en, "transcripción reversa y reacción de polimerasa en cadena"@es .

nalt:34216
  a ns0:Chemical, skos:Concept ;
  skos:prefLabel "DNA primers"@en, "cebadores de ADN"@es ;
  skos:related nalt:8124 .

nalt:343052
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "recombinase polymerase amplification"@en, "amplificación de recombinasa polimerasa"@es ;
  skos:related nalt:8124 .

nalt:292387
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "reacción en cadena de la hibridación"@es, "hybridization chain reaction"@en ;
  skos:related nalt:8124 .

nalt:275963
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "allelic dropouts"@en, "amplificación diferencial de los alelos"@es ;
  skos:related nalt:8124 .

nalt:275961
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "genotyping errors"@en, "errores de genotipado"@es ;
  skos:related nalt:8124 .

nalt:312563
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "análisis de la curva de fusión"@es, "melting curve analysis"@en ;
  skos:related nalt:8124 .

nalt:209544
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "hibridación del ácido nucleico"@es, "nucleic acid annealing"@en ;
  skos:related nalt:8124 .

nalt:17409
  a ns0:Topic, skos:Concept ;
  skos:narrower nalt:8124 ;
  skos:prefLabel "técnicas y protocolos genéticos"@es, "genetic techniques and protocols"@en .

nalt:226519
  a ns0:Topic, skos:Concept ;
  skos:broader nalt:8124 ;
  skos:prefLabel "ensayo de amplificación de mutación desajustada"@es, "mismatch amplification mutation assay"@en .

nalt:15977
  a ns0:Topic, skos:Concept ;
  skos:prefLabel "nucleotide sequences"@en, "secuencias de nucleótidos"@es ;
  skos:related nalt:8124 .